The Power of Shodex Columns for Light Scattering Detectors

Comparison of Baseline Noise with Using MALS Detector and LB-800 Series Columns

Signal of low molecular weight substances is relatively small, and it is difficult to detect the MALS signal due to the baseline noise with conventional SEC columns.  Shodex OHpak LB-800 series columns dramatically lower detection limits by virtually eliminating baseline noise.

Sample :  10 µL

Pullulan (MW : 12,000)

Columns: Shodex OHpak LB-806M, Shodex OHpak SB-806M HQ (8.0mm I.D. x 300mm each); SEC column from other manufacturer (7.8mm I.D. x 300mm each)

Eluent:  0.1M NaNO3 aq.

Flow Rate:  1.0 mL/min

Detector:  MALS (Multi angle light scattering) (90°)

Column Temp.:  30°C